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Electrochemical Immunosensing Chip Using Selective Surface Modification,Capillary‐Driven Microfluidic Control,and Signal Amplification by Redox Cycling |
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| Authors: | Byung‐Kwon Kim Sang‐Youn Yang Md Abdul Aziz Kyungmin Jo Daekyung Sung Sangyong Jon Han Young Woo Haesik Yang |
| Institution: | 1. Department of Chemistry, Pusan National University, Busan 609‐735, Korea tel: +82?51?510?3681;2. fax: +82?51?516?7421.:;3. Department of Life Science, Gwangju Institute of Science and Technology, Gwangju 500‐712, Korea;4. Department of Nanofusion Technology, Pusan National University, Miryang 627‐706, Korea |
| Abstract: | A sensitive electrochemical immunosensing chip is presented by employing (i) selective modification of protein‐resistant surfaces; (ii) fabrication of a stable Ag/AgCl reference electrode; (iii) capillary‐driven microfluidic control; (iv) signal amplification by redox cycling along with enzymatic reaction. Purely capillary‐driven microfluidic control is combined with electrochemical sandwich‐type immunosensing procedure. Selective modification of the surfaces is achieved by chemical reactivity‐controlled patterning and electrochemical deposition. Fluidic control of the immunosensing chip is achieved by spontaneous capillary‐driven flows and passive washing. The detection limit for mouse IgG in the immunosensing chip is 10 pg/mL. |
| Keywords: | Immunosensor Passive washing Microfluidic control Nonspecific binding Ag/AgCl reference electrode |