Fabrication of Bienzymatic Glucose Biosensor Based on Novel Gold Nanoparticles‐Bacteria Cellulose Nanofibers Nanocomposite |
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| Authors: | Wei Wang Hong‐Yi Li De‐Wen Zhang Jing Jiang Yi‐Ran Cui Shuang Qiu Ying‐Lin Zhou Xin‐Xiang Zhang |
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| Institution: | Beijing National laboratory for Molecular Sciences(BNLMS), Key Laboratory of Biochemistry and Molecular Engineering, College of Chemistry, Peking University, Beijing 100871, P.?R. China tel/fax: +86‐10‐62754680 |
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| Abstract: | An exploration of gold nanoparticles–bacterial cellulose nanofibers (Au‐BC) nanocomposite as a platform for amperometric determination of glucose is presented. Two enzymes, glucose oxidase (GOx) and horseradish peroxidase (HRP) were immobilized in Au‐BC nanocomposite modified glassy carbon electrode at the same time. A sensitive and fast amperometric response to glucose was observed in the presence of electron mediator (HQ). Both of GOx and HRP kept their biocatalytic activities very well in Au‐BC nanocomposite. The detection limit for glucose in optimized conditions was as low as 2.3 µM with a linear range from 10 µM to 400 µM. The biosensor was successfully applied to the determination of glucose in human blood samples. |
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| Keywords: | Au‐BC nanocomposite Horseradish peroxidase Glucose oxidase Biosensors Glucose |
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