An Amperometric Immunosensor for IgG Based on Conducting Polymer and Carbon Nanotube‐Linked Hydrazine Label |
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Authors: | Ye Zhu Wei Choon Alvin Koh Yoon‐Bo Shim |
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Institution: | 1. Department of Chemistry, Pusan National University, Keumjeong‐Ku, Busan 609‐735, Korea (ROK) tel.: (+82)?51‐510‐2244;2. fax: (+82)?51‐514‐2430 |
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Abstract: | An amperometric immunosensor for IgG was developed by covalently immobilizing anti‐IgG on multiwall carbon nanotube‐embedded conducting polymer, poly‐5,2′ : 5′′,2′′‐terthiophene‐3′‐carboxylic acid (MWCNT/pTTCA). The MWCNT/pTTCA modified electrode was characterized by SEM, EIS, and XPS. A hydrazine‐labeled secondary antibody‐MWCNT conjugate (Hyd‐MWCNT‐Ab2) was applied for detection. Hydrazine was used as a catalyst for the reduction of hydrogen peroxide, which was monitored at ?0.3 V vs. Ag/AgCl. The calibration plots showed a linear range of 0.1–10 ng/mL with a detection limit of 0.084±0.004 ng/mL. The proposed immunosensor was evaluated for clinical applications in a rabbit serum sample. |
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Keywords: | Carbon nanotube Conducting polymer Hydrazine label Immunoglobulin G Immunosensor |
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