单分子力谱研究活细胞上多药耐药相关蛋白1的表达

采用原子力显微镜的单分子力谱(SMFM)技术研究了多药耐药相关蛋白1(MRP1)与其抗体间的相互作用, 并考察了人舌癌细胞系TCA8113经高剂量平阳霉素(BLM)反复间歇诱导前后细胞表面MRP1的表达差异. 实验结果表明, MRP1与其抗体之间存在特异性相互作用力, 当针尖运动速率为2.5 μm/s时, 作用力大小约为(182±35) pN; 而且药物诱导后MRP1在人舌癌细胞上的表达明显增强. 本工作为了解活细胞水平上MRP1的表达提供了新方法, 有助于肿瘤细胞多药耐药性(MDR)的研究.

Investigation of MRP1 Molecules on Cell Membrane Based on Single Molecule Atomic Force Microscopy

Multidrug resistance(MDR) is one of the main factors in the failure of chemotherapy against tumors, and multidrug resistance-associated protein 1(MRP1) is closely related to the generation of MDR. In this work, the interaction force between MRP1 and anti-MRP1 was measured via single-molecule force microscopy(SMFM), and the expression differences of MRP1 on human tongue cancer cells before and after treatment with high-dose bleomycin(BLM) were investigated. The results show that the interaction force between MRP1 and anti-MRP1 is about (182±35) pN as the loading rate is 2.5 μm/s. The expression of MRP1 on human tongue cancer cell membrane increased obviously after treatment with high-dose BLM. This work may provide a new method for studying the expression of MRP1 at the living cell level and can be helpful for the study of MDR.