仿细胞膜结构共聚物的合成及涂层性能

采用饥饿法将2-甲基丙烯酰氧乙基磷酰胆碱(MPC)分别与甲基丙烯酸十八烷基酯(SMA)、 甲基丙烯酸十二烷基酯(LMA)及甲基丙烯酸正丁酯(BMA)聚合, 通过改变投料比例和沉淀剂种类, 合成了一系列含磷酰胆碱基团的仿细胞膜结构的两亲性二元随机共聚物. ¹H NMR和元素分析结果表明, 合成的两亲性二元随机共聚物的组成与投料比相近. DSC结果表明, 聚合物具有较低的玻璃化转变温度. 表面张力及水的动态接触角(DCA)研究发现, 聚合物涂层表面具有明显的两亲性及表面结构易变性, 在空气中憎水基团在表面取向, 在水环境中亲水的磷酰胆碱基团则迁移取向到涂层表面形成仿细胞外层膜结构界面, 最终形成不溶于水的仿细胞膜结构涂层.

Synthesis and Coating Properties of Biomembrane Mimetic Copolymers

2-Methacryloyloxyethyl phosphorylcholine(MPC), a hydrophilic monomer mimicking the cell outer membrane structure, was copolymerized with hydrophobic monomer stearyl methacrylate(SMA), lauryl methacrylate(LMA) and n-butyl methacrylate(BMA), respectively, by monomer-starved polymerization technique. By changing the molar ratio of the monomers in feed, a series of amphiphilic biomembrane mimetic copolymers bearing different hydrophobic alkyl chain groups was synthesized. The 1H NMR and elemental analysis results showed that the compositions of the amphiphilic random bipolymers were similar to the ratios in feed. The DSC results suggested that the glass transition temperature of these copolymers were low. The surface tension measurement indicated that the copolymer coatings rarely dissolved in water. The dynamic contact angles(DCA) of the copolymer coatings in water suggested a changeable surface structure of the coating. When exposed in air, the hydrophobic alkyl chains of the coating oriented onto the surface; while in aqueous conditions, the hydrophilic phosphorylcholine groups migrated and oriented onto the interface to form the cell outer membrane mimetic structure. This study provides theoretical support for improving the biocompatibility of biomaterials simply by immersing in or spraying with the solutions of the cell membrane mimetic amphiphilic copolymers.