Two-photon Lysotrackers for in vivo imaging |
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Authors: | Son Ji Hoon Lim Chang Su Han Ji Hee Danish Isravel Antony Kim Hwan Myung Cho Bong Rae |
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Affiliation: | Department of Chemistry, Korea University, 1-Anamdong, Seoul 136-701, Korea. |
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Abstract: | We report two-photon Lysotrackers (CLT-blue and CLT-yellow) that can be excited by 750-840 nm femtosecond laser pulses and emit at 470 and 550 nm, respectively. They can be easily loaded into cells and tissue slices for visualization of lysosomes in live cells and tissues for a long period of time through two-photon microscopy. When combined with appropriate two-photon probes for other biological targets, these novel probes would greatly facilitate the two-photon microscopy colocalization experiments. |
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