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Two-photon Lysotrackers for in vivo imaging
Authors:Son Ji Hoon  Lim Chang Su  Han Ji Hee  Danish Isravel Antony  Kim Hwan Myung  Cho Bong Rae
Institution:Department of Chemistry, Korea University, 1-Anamdong, Seoul 136-701, Korea.
Abstract:We report two-photon Lysotrackers (CLT-blue and CLT-yellow) that can be excited by 750-840 nm femtosecond laser pulses and emit at 470 and 550 nm, respectively. They can be easily loaded into cells and tissue slices for visualization of lysosomes in live cells and tissues for a long period of time through two-photon microscopy. When combined with appropriate two-photon probes for other biological targets, these novel probes would greatly facilitate the two-photon microscopy colocalization experiments.
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