透明质酸-量子点的制备及应用

基于量子点(QD)独特的光学成像特性, 采用化学合成法制备了透明质酸(HA)修饰的水溶性纳米量子点(HA-QD), 并将其应用于特异性受体CD44的识别研究中. 体外细胞实验结果证实, 在透明质酸受体的介导下, 该纳米复合物可使小鼠肺腺癌细胞LA795显示特异性的荧光成像. 本研究为建立针对透明质酸受体的肿瘤活体检测及研究肿瘤的发生发展提供了重要的纳米靶向荧光探针.

Preparation of Hyaluronic Acid-Quantum Dot Conjugate and Its Application in Tumor Imaging

Hyaluronic acid(HA) has been known as an important element in tumor growth,because its receptor,CD44,is over expressed on many kinds of tumor cells.Thus,the interaction between HA and CD44 has been used for HA-targeted therapy and imaging of malignancy.In order to establish a method to detect the HA receptor in the cell and further to image the tumor cell,hyaluronic acid-quantum dot conjugate was synthesized and applied in tumor imaging.The quantum dot(CdSe/ZnS) was prepared in aqueous solution with cysteamine as stabilizer,and then conjugated with HA through amidation reaction between the carboxyl groups of HA and amino groups of QD.The average size of HA-QD conjugate nanoparticle was about 10 nm and emission wavelength was 657 nm.According to in vitro cell experiment,it was proved that the HA-QD conjugate could effectively target on the LA795 cells and subsequently internalized itself into the cytoplasm via HA receptor-mediated endocytosis.As its cancer targeting efficiency showing in our experiment,it suggests the potential of HA-QD conjugate applying in imaging and visualization of changes for cancer diagnosis.