Applications of the ring oven method in enzymatic analysis

The use of the ring oven technique in enzymatic analysis is described. The “segment technique” for catalyzed reactions is applied for the determination of enzymes, inhibitors and substrates. Three different methods have been worked out for this purpose, none of them needs a stable standard scale. These methods are illustrated by various examples: determinations of the enzymes acid and alkaline phosphatase, hyaluronidase, acetylcholinesterase, lipase and alcohol dehydrogenase; indirect determinations of the inhibitors copper, beryllium, molybdenum, the insecticides carbaryl and paraoxon, the herbicides 2, 4-D, mecoprop and bentazcn, and the fungicide phenylmercury acetate; and determinations of the substrates ethanol and glucose.