The double radio-isotope derivative technique for the assay of drugs in biological material: Part II. The simultaneous determination of opipramol and its deshydroxyethyl metabolite |
| |
Authors: | W. Riess |
| |
Affiliation: | Research Department, Pharmaceuticals Division, CIBA-GEIGY Limited, Basle Switzerland |
| |
Abstract: | The neuroleptic drug opipramol and its deshydroxyethyl metabolite can be determined simultaneously in the same biological sample. Known amounts of 14C-labelled opipramol and 14C-labelled metabolite are added to the sample to serve as internal standards. After suitable extraction, both compounds are acetylated by 3H-labelled acetic anhydride. Together with μg-amounts of carrier compounds, the O-acetyl derivative of opipramol and the N-acetyl derivative of the metabolite are purified and separated by two-dimensional thin-layer chromatography. Each of the derivatives is isolated and counted for 14C-and 3H-activity. The 14C-activities recovered serve to determine the overall yield of the opipramol and metabolite, and to convert the measured 3H-activity to 100 % theoretical yield. From analyses of standard samples, the specific 3H-activities of the acetyl derivatives were calculated, and these values were used to convert the measured 3H-activities from biological samples to concentrations of original opipramol and metabolite. For both compounds the standard deviations of blank samples were ±1 ng ml?1. For concentrations up to 100 ng ml?1 the standard deviation was ±3 ng ml?1. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|