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| 快速溶剂萃取-离子色谱法测定茶叶中的有机酸 | |
| 引用本文： | 章剑扬,刘新,车金水,汪琼,陈艳,叶明立. 快速溶剂萃取-离子色谱法测定茶叶中的有机酸[J]. 化学分析计量, 2012, 21(1): 43-45 |
| 作者姓名： | 章剑扬 刘新 车金水 汪琼 陈艳 叶明立 |
| 作者单位： | 1. 中国农业科学院茶叶研究所,杭州,310008 2. 赛默飞世尔有限公司上海实验室,上海,201203 3. 浙江省环境保护科学设计研究院,杭州,310007 |
| 摘 要： | 建立了快速溶剂萃取-离子色谱法（ASE—IC）测定茶叶中的9种有机酸。优化了ASE苹取条件，以水为萃取溶剂，80℃下萃取5min，循环两次。采用Ionpac AS11HC柱分离，KOH溶液梯度洗脱，流速1,0mL／min，抑制电导检测。9种有机酸的检出限为0．009-0．160μg／mL，线性范围为0．5—10μg／mL，相关系数大于0．999，加标回收率为93．4％-99．2％，相对标准偏差为1．4％-4．1％。 |
| 关 键 词： | 快速溶剂萃取 离子色谱 有机酸 茶叶 |
Determination of Organic Acid in Tea by Accelerated Solvent Extraction-Ion Chromatography |
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| Zhang Jianyang , Liu Xin , Che Jinshui , Wang Qiong , Chen Yan , Ye Mingli. Determination of Organic Acid in Tea by Accelerated Solvent Extraction-Ion Chromatography[J]. Chemical Analysis And Meterage, 2012, 21(1): 43-45 | |
| Authors: | Zhang Jianyang Liu Xin Che Jinshui Wang Qiong Chen Yan Ye Mingli |
| Affiliation: | 1.Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China; 2.Shanghai Lab, Thermofisher Scientific, Shanghai 201203, China; 3.Environmental Science Research and Design Institute of Zhejiang Province, Hangzhou 310007, China) |
| Abstract: | A method was developed for the determination of nine organic acids （OA） in tea by accelerated solvent extraction-ion chromatography （ASE-IC）. The ASE extraction conditions were optimized. The tea samples were extracted at 80℃, for 5 rain and two extraction cycles using water as extractant. The Dionex Ionpac AS11 HC was employed as analytical column, KOH solution as the gradient elution at a flow rate of 1.0 mL/min, and a suppressed conductive detector as the detector. The method obtained good linearity in the range of 0.5-10 μg/mL for organic acids with relative coefficient more than 0.999. The detection limits were 0.009-0.160μg/mL （S/N=3）, the recoveries of the standard addition experiment ranged from 93.4%-99.2%, and the relative standard deviation ranged from 1.4% to 4.1%. |
| Keywords: | accelerated solvent extraction ion chromatography organic acid tea |
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