径向电场调制毛细管电泳法用于神经递质分离

利用双向电场控制毛细管电泳系统，考察了神经递质的分离。在pH2.5的0.01mol/L磷酸盐缓冲体系中，通过加入20％（V／V）正丙醇，改善了多巴胺和5－羟色胺的分离效果，但仍不太理想。通过施加径向电场，可进一步提高分离度。本研究不仅拓宽了径向电场调控的样品分离范围，而且为生物活性物质的痕量分析提供了参考依据。

Capillary Electrophoresis of Neurotransmitters Under the Control of Radial Electric Field

Separations of neurotransmitters such as dopamine (DA), serotonin (5-HA), norepinephrine (NE) and epinephrine (E) were performed successfully using a homemade electric field modulated capillary electrophoretic system, which could offer both radial and axial electric fields with only one high voltage power supply. DA and 5HT were eluted simultaneously and could not be resolved in 0. 01 mol/L phosphate buffer at pH 2. 5. Alcohol additives, such as methanol, ethanol or 1-propanol were added to the buffer to change the solvation shell of the solutes, which changed their effective sizes and electrophoretic mobilities of the solutes accordingly. The optimum composition was a buffer of 20% (V/V) 1-propanol, with resulted resolutions 0.74 (DA/5-HT), 0. 56(5-HT/NE) and 0.77 (NE/E). If a positive radial voltage of 6.6 kV was applied, the resolutions were improved to 1 .48, 0.71 and 1. 32, respectively.