Semipreparative High-Pressure Liquid Chromatography of Synthetic Carbohydrates

Abstract

A rapid and effective method has been developed for the purification of hundred-milligram quantities of water-soluble synthetic carbohydrates. The method utilizes a 30 cm x 7.8 mm i.d. column packed with Waters Associates carbohydrate analysis packing in conjunction with isocratic water-acetonitrile elution and refractive index detection. The generality of this method is illustrated by its application to 13 sucrose derivatives and one derivative each of D-fructose and α,α-trehalose.