A Simultaneous Analysis by High Performance Liquid Chromatography of Bamipine Combined with Tricyclic Antidepressants and/or Antipsychotics in Dosage Forms

Abstract

A reversed phase high-performance liquid chromatographic method is described for the simultaneous determination of antihistamines, tricyclic antidepressants and anti-psychotics in pharmaceutical formulations and in spiked placebos. The separation was performed on an octadecyl-silica column using acetonitrile: tetrahydrofuran: 0.015 M aqueous ammonium acetate (53:42: 5) as mobile phase. The presence of ammonium acetate both shortens the elution time and improves the symmetry of the chromatographic peaks. Measurements were made at 251 nm.