Ultramicro Analysis of Reducing and Non-Reducing Sugars by Liquid Chromatography

Abstract

A post-column detection system for ultramicro amount of sugars has been developed using taurocyamine as the labelling reagent. Less than 10 pmol of reducing sugars were determined by this HPLC system. Non-reducing sugars were detected by the addition of periodate to the reagent.

Modification of the reaction reagent components made this detection system feasible to apply to various methods for separation of carbohydrates using pure water, acetonitrile/water mixtures, borate buffer or aqueous sodium hydroxide as the eluent.