Characterization and Properties of Macromolecules. Part X. High Speed Aqueous Gel Permeation Chromatography Using Hydrogel Column Packing

Abstract

Hydrogel, a commercially available column packing, has been evaluated for high speed aqueous gel permeation chromatography. The commercial material designated as < 44μ particle size was classified into narrow particle size ranges using a sonic sifter. The pore volume was found to be independent of particle size. Efficiency was not found to be dependent upon particle size when these classified materials were dry packed. The application of Hydrogel to determine molecular weight distribution of a variety of water-soluble polymers was examined.