Separation of a Homologous Series of Long-Chain Fatty Alcohols (C49-C58) From Mycobacterium Tuberculosis H37Ra by High Performance Liquid Chromatography

Abstract

Fatty alcohols were obtained from the saponified chloro-form-methanol extract of Mycobacterium tuberculosis H37Ra by extraction with diethyl ether and partially purified by silicic acid column chromatography. The long-chain fatty alcohols (C₄₉-C₅₈) were separated from the shorter-chain alcohols by Sephadex LH-20 column chromatography and further fractionated into saturated and unsaturated alcohols by argentation thin-layer chromatography. These two fractions were analyzed by proton nuclear magnetic resonance spectro-scopy, derivatized to the 3,5-dinitrobenzoyl esters, and fractionated on a C₁₈-bonded silica column by high performance liquid chromatography (HPLC). Complete separation of esters differing by two carbon units and partial separation