Effect of Type and Concentration of Organic Modifier in Aqueous Eluents on Retention in Reversed Phase Systems

Abstract

Retention - eluent composition relationships were determined for 18 compounds (phenols, quinoline bases, anilines) in chromatographic systems of water + organic modifier - oleyl alcohol (fixed on cellulose). Six organic modifiers were investigated: methanol, acetone, acetonitrile, 1,4-dioxane, dimethyl sulphoxide, acetic acid. Different eluotropic series were obtained depending upon the donor-acceptor properties of the solutes; for instance, dimethyl sulphoxide exhibited high eluent strength relative to phenols and anilines and acetic acid - to quinolines and anilines.