High Performance Liquid Chromatographic Analysis of Penicillin V Solid Dosage Forms

Abstract

An isocratic high performance liquid chromatographic (HPLC) method for the determination of Penicillin V in solid dosage forms is described. A reverse phase RP-8 column and a mobile phase of 52% methanol in 0.05 M phosphate buffer (pH 3.3) were employed. Detection was effected at 254 nm. The results obtained are compared with those from the iodometric method.