High Performance Liquid Cbromatographic Separation of Globin Chains on a Large-Pore C4 Column

Abstract

Excellent resolution of human and baboon globin chains may be obtained by HPLC on a Vydac large-pore C₄ column. The procedure is rapid and uses a gradient between aqueous trifluoroacetic acid and trifluoroacetic acid in acetonitrile. The common human γ chains are easily separable from each other as are some α- and β-chain variants from the normal chains and from each other.