Urinary Uric Acid Determination by Reversed-Phase High-Performance Liquid Chromatography with Electrochemical Detection |
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Abstract: | Abstract Determination of urinary uric acid has been attempted by reversed-phase high-performance liquid chromatography with electrochemical detection. We have found that the electrochemical detection method is suitable for monitoring eluate from reversed-phase column and also that the minimum detectable quantity of uric acid using en electrochemical detector is about 10 pg. Complete separation of uric acid was achieved in about 8 min under the present chromatographic conditions. |
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