Determination of Piperacillin in Plasma and Cerebrospinal Fluid by High Performance Liquid Chromatography

Abstract

Reversed-phase high performance liquid chromatography was applied to the quantitative determination of a new β lactam antibiotic piperacillin. The procedure was applied to human plasma and cerebrospinal fluid samples. After an extraction of plasmaor cerebrospinal fluid by dimethyl formamide, chromatographic separation was effected on a bonded hydrophobic stationary phase with one mobile ohase: methanol-phosphate buffer. This assay was applied to study human pharmacokinetics on ten patients with purulent meningitis during their therapy. Simultaneous determination of piperacillin in plasma and cerebrospinal fluid one and two hours after piperacillin perfusion showed a good penetration of this antibiotic in human cerebrospinal fluid.