Simultaneous High Performance Liquid Chromatographic Determination of Amitriptyline Hydrochloride and Perphenazine in Tablet Formulations

Abstract

A rapid, precise, and accurate high performance liquid chromatographic procedure is presented for the simultaneous determination of amitriptyline hydrochloride and perphenazine in two component tablet formulations. The related compounds of amitriptyline hydrochloride were separated, making the determination specific for amitriptyline hydrochloride and perphenazine. The method was used for the assay and content uniformity for three commercial products. The mobile phase was 0.02 M ammonium acetate in aceto-nitrile: methanol: water (45:15:40) solution and the pH was adjusted to 5.0 by acetic acid. The column was a supelcosil (5 μm) LC-8-DB (250 mm × 4.6 mm i. d). The method was tested for linearity, recovery, and specificity.