HPLC Separation and Characterization of Synthetic and Native Cytochrome C Derived Peptides

Abstract

The use of HPLC for the characterization of peptides used in semisynthesis of cytochrome c is discussed. HPLC provided a convenient method for assessing the purity and structure of the peptides used. HPLC was also useful in monitoring the progress of solid phase peptide synthesis and in purifying the final product.