Cleanup of Samples for High Performance Chromatography and Instrumental Analysis

Abstract

Cleanup methods have been developed which allow utilization of modern chromatographic techniques and instrumental readouts. A cellulose cleanup for organo-phosphorus pesticides and carbamates has already been reported. A particulate carbon column has been described for Rumensin. These columns maybe combined to improve the cleanup for organophosphorus and carbamate pesticides. This same column cleanup maybe used for Ochratoxin. Powder charcoal maybe used for the mycotoxins aflatoxin, and zearalencne. It maybe used for T-2 toxin and trichothecenes with some loss (50-75% recovery).

The thin layer plates themselves provide a background reference for spectroscopy. This background maybe removed by “annealing” the plates at 400°C for 2 hours.

Sweepco distillation maybe used to cleanup biological samples so that carbamates and organophosphorus pesticides maybe analyzed by thin layer chromatography. Trichothecenes mycotoxins maybe cleanuped with about 40% loss.