Rapid Analysis of 6-Diazo-5-oxo-L-norleucine (DON) in Human Plasma and Urine

Abstract

A paired-ion, reversed phase high pressure liquid chromatography (HPLC) procedure is described for the analysis of DON in human plasma and urine. Plasma proteins are removed by centrifugal membrane filtration, and the filtrate is injected directly onto an octadecylsilane column. The DON is eluted in a mobile phase consisting of 5 mM 1-heptanesulfonic acid, pH 2.4. Eluting material is monitored at 280 nm and 254 nm. The lower limit of sensitivity in plasma is 0.1 μg/ml.