The Determination of Ioglycamic Acid in Bile and in Urine by High Performance Liquid Chromatography

Abstract

A fast, simple and specific method for the determination of ioglycamic acid in bile and in urine is described. Reversed-phase ion-pair chromatography with methanol-water (67.5: 32.5) in the presence of the counter ion tetrabutyl-ammonium phosphate as the mobile phase separates ioglycamic acid in 5 min at a flow rate of 1 ml/min. Under the same conditions urinary creatinine can be simultaneously determined and may be used as an endogenous internal standard. Bile and urine from a healthy subject were screened for possible metabolites but these are not detected.