Measurement of S-2-(3-Aminopropylamino)ethanethiol (WR1065) in Blood and Tissues

Abstract

A procedure for the analysis of blood and tissue specimens for WR1065, the dephosphorylated metabolite of the radioprotective drug WR2721, has been developed. The method includes the use of a perchloric acid/EDTA extraction step at 0°C followed by chromatographic analysis using a mercury/gold thin film electrochemical detection liquid chromatography system. The extraction technique was designed to assure the stability of both WR1065 and any WR2721 present in blood or tissues. Using the described chromatography conditions and an analog of WR1065, 3-(4-aminopropylamino)propanethiol (WR251833), as an internal standard the respective retention times of these two compounds are 6.2 and 8.3 minutes. Experiments showing the applicability of this method to pharmacokinetic studies of WR2721 and WR1065 and to investigation of the kinetics of WR2721 hydrolysis in biological fluids such as stomach juice are described.