Determination of Sterigmatocystin in Fungal Cultures by High Pressure Liquid Chromatography

Abstract

Levels of sterigmatocystin in cultures of various fungal strains were determined by high pressure liquid chromatography (HPLC). This mycotoxin was well separated using acetonitrile / water / acetic acid (55/45/2) as solvent on a μ-Bondapak C18 column (Waters Associates). It was detected by its absorbance at 320 nm. Using this method, sterigmatocystin can thus be simply and rapidly determined in mould strain cultures.