High-Performance Liquid Chromatography of New Semisynthetic Daunomycinone Derivatives

Abstract

Reversed-phase HPLC utilizing LiChrosorb RP-8 was used to separate reaction mixtures of new semisynthetic daunomycinone derivatives and determine their relative occurrence.

Chromatographic behaviour of the following compounds was studied: daunomycinone (I), 7(S) and 7(R)-0-(2-hydroxyethyl)-13-ethyleneacetal daunomycinone (II and III), 13-ethyleneacetal daunomycinone (IV), 13-ethyleneacetal bisanhydrodaunomycinone (V), 7(S) and 7(R)-0-(3-hydroxypropyl)-13-propyleneacetal daunomycinone (VI and VII), 13-propyleneacetal daunomycinone (VIII), 13-propyleneacetal bisanhydrodaunomycinone (IX), 7(S) and 7(R)-0-(4-hydroxybutyl) daunomycinone (X and XI), 4-toluenesulfonylhydrazone daunomycinone (XII).