Linear Multidimensional Liquid Chromatography in Protein Separation

Abstract

The objective of this article is to define, discuss and illustrate the concept of on-line multidimensional liquid chromatography (MDLC) in protein separation. In particular the emphasis of this paper is centered on a special form of on-line MDLC that will be referred to as linear MDLC. Examples of this technique, which involves the coupling of two or more chromatographic columns each employing a different separation mechanism, in both the analytical and preparative mode, are given in order to demonstrate its utility.