Synergistic Use of Countercurrent Chromatography and High Performance Liquid Chromatography for the Purification of Synthetic Peptides

Abstract

Examples are given demonstrating how countercurrent chromatography (CCC) and high performance liquid chromatography (HPLC) can be used together to purify synthetic peptides. In one example, CCC provided a preliminary purification of Met-Arg-Asp-Val-Val-Leu-Phe-Glu-Lys by enabling separation of ultraviolet absorbing, ninhydrin-negative material from the desired peptide. Final purification was achieved with HPLC without risk of harming the HPLC column. In a second example Tyr-Ala-Ala-Nle-Ala-Ala was completely purified by CCC with the CCC separation rapidly and conveniently monitored by HPLC. CCC appears to be a very useful technique for the peptide chemist.