Determination of Penicillins and Cephalosporins N-Pyrrolylderivatives by HPLC

Abstract

An isocratic reversed-phase liquid chromatographic method for the determination of different types of penicillins and cephalosporins N-pyrrolylderivatives using UV detection at 254 nm is described. The mobile phases were aqueous methanolic (40–60%, v/v), 0.05M potassium phosphate buffer, pH 6.0.

The method is sufficiently sensitive and precise and is thus highly suited for use in the kinetic studies.