HPLC Determination of Monoamines in Rat Brain after Enzymatic Treatment with Ascorbate Oxidase and Sulfatase |
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Abstract: | Abstract The simultaneous analysis of norepinephrine, dopamine and serotonin along with their respective metabolites, MHPG, DOPAC and 5HIAA is accomplished in rat brain by using a recently developed HPLC technique. Sulfated MHPG is decongegated with sulfatase for detection purposes and endogenous ascorbate is diminished with ascorbate oxidase to reduce the front. |
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