Solid Phase Extraction and HPLC Analysis of Tryptamide in Plasma

Abstract

A method for extraction and quantification of tryptamide in plasma is described in this report. The method employs Amberlite XAD-2 column extraction followed by HPLC with ultraviolet detection. The procedure is simple, rapid and reproducible. It has been applied to the measurement of tryptamide in plasma of rats dosed orally with this antiinflammatory and analgesic compound.