Practical Microbore Column HPLC: System Development and Drug Applications

Abstract

Commercially available packed microbore columns have been used to demonstrate the potential of microbore high performance liquid chromatography (micro-LC) systems. The necessity of optimizing the chromatographic system for micro-LC is demonstrated using sulfa drugs and antibiotic standards, and some of the advantages of micro-LC such as high mass sensitivity are shown. Finally micro-LC has been used for the determination of two drugs, reserpine and trichlormethiazide in biological fluids.