Application of High Pressure Liquid Chromatography to the Forensic Analysis of Several Benzodiazepines

Abstract

Eleven benzodiazepines are separated and measured by an HPLC adsorption system utilizing isocratic elution combined with dual wavelength detection at 254 nm and 280 nm. Powder and solid dosage forms of 10 benzodiazepines are simply ground and then extracted with chloroform and injected. Clorazepate dipotassium is not soluble in chloroform and is decarboxylated to N-desmethyldiazepam.