A HPLC Method for the Determination of Butaperazine in Solutions,Tablets, Plasma and Bile

Abstract

A specific HPLC method for the determination of the antipsychotic drug butaperazine (B) in solutions, tablets, plasma and bile has been developed. The instrument used was a Waters HPLC equipped with a Model 440 Spectrometer and a μ-Bondapak-NH₂ column. The mobile phase, chloroform-methanol (100:3.5) was pumped at a rate of 1.5 ml per minute. Ultraviolet absorbance at a wave length of 280 nm was used for detection. The procedure involved the extraction of the drug from the dosage forms with chloroform and from plasma and bile with hexaneisopropanol (9:1). Hydrocortisone acetate was used as the internal standard. Retention times of 2.4 and 3.9 minutes were obtained for the internal standard and B respectively. Analytical calibration yielded a linear relationship from 0.1–25 μg per ml, with r² value of 0.99. The percentage recovery of B averaged 99% from the dosage forms and 94% from the biological fluids. An improvement in the method for determining B in bile and plasma was later developed. It involved the use of a different mobile phase and detecting the drug fluorometrically.