Biological Characterization of a Pituitary Monkey Lutropin Preparation after Chromatofocusing or after High Performance Anion Exchange Chromatography

Abstract

The Pharmacia Fast Protein Liquid Chromatography System equiped with a Mono P HR 5/10 chromatofocusing column was used to characterize the distribution of different bioactive forms of a cynomolgus (Macaca bascicularis) pituitary lutropin preparation. The results were compared with the profiles obtained after running the preparation on a Waters DEAE anion exchange column using eluants in the pH range from 6.0 to 8.0. The distribution of preserved biological activity were studied in respective eluted fractions from the different experiments of the applied preparation. Seventy-six percent of the bioactivity was recovered after chromatofocusing whereas preserved bioactivity after ion exchange chromatography ranged between 61–78% at pH 6.5 to 7.5. No bioactivity was restored after elution at pH 6.0 or 8.0.