A Simple High Performance Liquid Chromatography Procedure for the Determination of N1-Methylnicotinamide in Urine

Abstract

A simple high performance liquid chromatography procedure for the determination of N¹-methylnicotinamide in urine samples is described. The procedure eliminates the need for extraction or ion exchange clean-up of urine samples prior to their analysis. Human and rat urine samples can be analyzed for N¹-methylnicotinamide directly following a simple pH adjustment. The metabolite was separated and quantitated on a 5μ Ultrasphere ODS (C18) reverse-phase column. The mobile phase contained 10 mM K₂HPO₄ and 10 mM sodium 1-octanesulfonate in 8% acetonitrile at pH 7.0. The system has been used to conduct over 1000 determinations during a period of three months without reduction in performance or efficiency.