Selective Separation of Polar Compounds Using an Electric Field Coupled Normal Phase HPLC Column

Abstract

An electric field coupled method for separation of polar compounds using normal phase high performance liquid chromatography is described. The method, which is not based on electrophoresis or electrochromatography, selectively changes the column capacity for polar analytes. The use of different columns showed improved column efficiency and up to a 62% increase in the number of theoretical plates. The theory for the improvement is presented. The significance and utility of the method for selective analysis of polar fractions in environmental samples is discussed.