Determination of Aztreonam by liquid Chromatography with UV and Amperometric Detection

Abstract

A comparative study of UV and amperometric detection of aztreonam after HPLC separation is presented. At pH 2.0 and a detection potential of +1.15 V (vs. Ag/AgCl), the detection limits with amperometric detection are about two times higher (3–5 ng) than those obtained with UV detection (1–3 ng) for aztreonam and its main decomposition products, the E-isomer and open-ring aztreonam. With the advantage of specificity for the aminothiazole group of the aztreonam molecule, amperometry can be used as an alternative or complementary mode to UV detection for the determination of aztreonam in injectable formulations and in human serum.