Systematic Liquid Chromatographic Separation of Poly-, Oligo-, and Monosaccharides

Abstract

High speed separations of poly-, oligo-, and monosaccharides were achieved on Chromosorb LC 9, Toyo Soda Starch Gel TSKLS17OP5, and Hitachi 3013N packings. Post column reaction system with tetrazolium blue was used to obtain a low detection limit for oligo-, and monosaccharides. Polysaccharides were analysed within 20 min by gel permeation chromatography over TSKLS17OP5 gel. DP 30 oligosaccharide was eluted in 30 min on Chromosorb LC 9 using a gradient of acetonitrile/water. Finally monosaccharides were separated in 25 min on Hitachi 3013N macroporous anion exchange resin.