An Improved Semi-Preparative Liquid Chromatography Technique to Purify A Uremic Toxin Fraction Inhibiting Na+, K+-Atpase

Abstract

We reported previously inhibition of Na⁺,K⁺-ATPase by a fraction (fraction 2–3) of medium size uremic toxins. Due to considerable amount of sulfates in this fraction, a semi preparative high performance liquid chromatography turned out to be inadequate to isolate the active compound. Therefore we developed a novel chromatographic method using ion exchanger Sephadex QAE A25 in acidic medium. This method allows the elimination of sulfates and the partial purification of the active component.