High-Pressure Liquid Chromatographic Analysis for Quantitation of BMY-28142 and Ceftazidime in Human and Rabbit Serum

Abstract

The quantitative measurement of BMY-28142 and ceftazidime in serum after protein precipitation were compared using a reverse-phase liquid chromatographic (LC) method. Detection using ultraviolet absorbance at 275 nm gave a sensitivity of 1.0 mcg/ml and 1.5 mcg/ml for BMY-28142 and ceftazidime respectively, and both gave linear response to concentration of at least 80 mcg/ml. Drug recovery after sample deproteination was 104% for BMY-28142 in rabbit serum and 93% in human serum. Results indicate a precise and accurate assay for this drug can be obtained using a simple LC method.