Fluorogenic Labelling of Carbonylcompounds with 7-Hydrazine-4-nitrobenzo-2-oxa-1,3-diazole (NBD-H)

Abstract

A method for the prechromatographic fluorescence derivatization of carbonyl compounds with 7-hydrazino-4-nitrobenzo-2-oxa-1,3-diazole (NBD-H) is presented. The separation and quantitation of the hydrazones is carried out by TLC and HPLC on silica gel and RP-ma-terials. Detection limits obtained for benzaldehyde by TLC with fluorodensitometric evaluation are 5 ng/spot and by HPLC with fluorescence detection 200 pg.