Elution Behavior of Low Molecular Weight Compounds in Gel Permeation Chromatography

Abstract

Elution behavior of organic compounds in gel permeation chromatography was investigated using chloroform as eluent. In aliphatic hydrocarbons, the elution counts decreased linearly with increasing the molecular volumes. In aromatic hydrocarbons, the relation between molecular volume and elution count slightly shifted toward lower counts. The elution counts in esters, ketones, amides, alcohols and carboxylic acids always fell in lower elution counts than expected by aliphatic hydrocarbons. This fact suggests that all these compounds are solvated by eluent molecules. Amines and chlorides exhibit an adsorption effect on cross-linked polystyrene gel. These compounds are eluted behind the corresponding hydrocarbons for given molecular volumes, which were obtained by dividing molecular weight by density.