Differential Binding of Sugars and Polyhydric Alcohols to Ion Exchange Resins: Inappropriateness for Quantitative HPLC

Abstract

The use of hydroxyl exchange resins (Dowex AG 1-X8 and AG 501-X8) as a component in the preparative clean up of biological samples for HPLC sugar and polyhydric alcohol analysis is inappropriate. On a weight specific basis, these resins bind 95–100% of monosaccharides (fructose and glucose), 45–85% of disaccharides (sucrose and trehalose) and 15–50% of polyhydric alcohols (glycerol and adonitol) present in sample solutions.