Liquid Chromatographic Determination of Quazepam in Commercial Tablets

Abstract

A simple and rapid reverse-phase liquid chromatography method is described for quantification of quazepam in tablets. Quazepam is extracted with methanol. An aliquot (10 ul) of the diluted methanolic extract was injected into the chromatograph. Chromatographic separations were made on a Adsorbosphere C₈ column (10 cm × 4.6 mm I. D.). Chromatograph was operated at ambient temperature with a mobile phase of 0.002 M phophate buffer (pH 4.0)-methanol (40:60) using a flow rate of 1.5 ml/min. Effluents were monitored at 265 nm. Retention times for diazepam (internal standard) and quazepam were 5.41 min and 8.67 min, respectively. Excellent day-to-day reproducibility of the slope of the standard curve and recovery data were obtained.