Structural Investigation of the Antibiotic Sporaviridin. XV. Preparative-Scale Preparation of Sporaviridin Components by HSCCC

Abstract

Preparative-scale separation of the six sporaviridin components, very complicated basic glycoside antibiotics produced by Streptosporangium viridogriseum, was carried out by high speed countercurrent chromatography (HSCCC). The following two points, selection of proper solvent system and stationary phase retention, were carefully examined. Many two-phase solvent systems were searched, so that a solvent system, n-butanol:diethylether:water =10:4:12, was selected and other operating conditions were also optimized. The HSCCC was performed under the optimized conditions and gave satisfactory results.